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51.
G. T. Fouad S. Servidei S. Durcan E. Bertini L. J. Ptácek 《American journal of human genetics》1996,59(1):135-139
Dyskinesias are hyperkinetic and involuntary movements that may result from any of a number of different genetic, infectious, and drug-induced causes. Some of the hereditary dyskinetic syndromes are characterized by paroxysmal onset of the abnormal movements. The classification of the familial paroxysmal dyskinesias (FPD) recognizes several distinct, although overlapping, phenotypes. Different forms of the disorder include attacks that are (1) induced by sudden movement (kinesiogenic); (2) spontaneous (non-kinesiogenic); and (3) induced by prolonged periods of exertion. Linkage analysis was pursued in a family segregating an autosomal dominant allele for non-kinesiogenic FPD. The disease allele was mapped to a locus on chromosome 2q31-36 (LOD score 4.64, theta = 0). Identification of distinct genetic loci for the paroxysmal dyskinesias will lead to a new genetic classification and to better understanding of these disorders. 相似文献
52.
The N-acetyl-beta-D-hexosaminidase B of germinating Lupinus luteus L. seeds (McFarlane et al. (1984) Phytochemistry 23, 2431-2433) was partially purified with a six-step purification procedure following extraction. This enzyme consists of one protein chain (Mr 69,000, as determined by SDS-PAGE and 62,500, as obtained by gel filtration on Bio-Gel P-60 Gel) and has a neutral isoelectric point (pI = 7.05, as determined by chromatofocusing). Moreover, it was found to be very sensitive to low ionic strength, especially in the presence of different gels based on Sephadex. Considering the substrate specificity, the enzyme splits both p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucosaminide and -galactosaminide substrates, but lacks N,N'-diacetylchitobiase activity. A new mixed-substrate procedure was developed and is presented here to demonstrate that a common active site is responsible for the splitting of both synthetic substrates. 相似文献
53.
Helices are the most common elements of RNA secondary structure. Despite intensive investigations of various types of RNAs, the evolutionary history of the formation of new helices (novel helical structures) remains largely elusive. Here, by studying the nuclear ribosomal Internal Transcribed Spacer 2 (ITS2), a fast-evolving part of the eukaryotic nuclear ribosomal operon, we identify two possible types of helix formation: one type is “dichotomous helix formation”—transition from one large helix to two smaller helices by invagination of the apical part of a helix, which significantly changes the shape of the original secondary structure but does not increase its complexity (i.e., the total length of the RNA). An alternative type is “lateral helix formation”—origin of an extra helical region by the extension of a bulge loop or a spacer in a multi-helix loop of the original helix, which does not disrupt the pre-existing structure but increases RNA size. Moreover, we present examples from the RNA sequence literature indicating that both types of helix formation may have implications for RNA evolution beyond ITS2. 相似文献
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Unravelling the coordination between leaf and stem economics spectra through local and global scale approaches 下载免费PDF全文
Sebastián R. Zeballos Melisa A. Giorgis Marcelo Cabido Diego E. Gurvich 《Austral ecology》2017,42(4):394-403
The existence of a coordination between leaf and stem economic spectra in woody species has been postulated repeatedly in the literature, with contrasting results. Here, we postulated that this coordination is conditioned by climate factors, being stronger in stressful environments. To test this hypothesis we explored the coordination between leaf and stem economic spectra in a seasonally dry forest in central Argentina and at the global scale, we analysed if the outcome of their coordination varies along a climatic gradient. At the local scale, we characterized leaf and stem economic spectra in 37 woody species by measuring six leaf and stem functional traits related to resource acquisition and use, and two functional traits used as proxies of water transport and use capacities. At the global scale, a meta‐regression was performed to analyse if the outcome of the coordination among leaf and stem traits varies along gradients of the mean precipitation of the driest quarter and of the minimum temperature of the coldest month. At the local scale, we observed a high integration among the measured leaf and stem traits, and this coordination seemed to be linked to hydraulic properties. At the global scale, we found not only that the overall weighted mean effect size of the correlation between specific leaf area and wood density was significant and negative but also that the coordination between leaf and stem traits seemed to be shaped by climate and tends to become stronger under harsh climate conditions. Furthermore, although our results seem to suggest that their coordination is context‐dependent, alternative strategies could be observed under stressful conditions. 相似文献
56.
Thais I. S. Riback Nildimar A. Honório Renato N. Pereira Wesley A. C. Godoy Cláudia T. Code?o 《PloS one》2015,10(8)
This study focuses on two competing species, Aedes aegypti and Aedes albopictus (Diptera: Culicidae), both invasive mosquitoes of the New World. Context-specific competition between immature forms inside containers seems to be an important determinant of the coexistence or displacement of each species in different regions of the world. Here, competition experiments developed at low density (one, two or three larvae) and receiving four different resource food concentration, were designed to test whether Ae. albopictus and Ae. aegypti respond differently to competition, and whether competition can be attributed to a simple division of resources. Three phenotypic traits - larval development, adult survival under starvation and wing length - were used as indicators of performance. Larvae of neither species were limited by resource concentration when they were alone, unlike when they developed with competitors. The presence of conspecifics affected Ae. aegypti and Ae. albopictus, inducing slower development, reduced survival and wing length. The response to resource limitation was different when developing with heterospecifics: Ae. aegypti developing with one heterospecific showed faster development, producing smaller adults with shorter lives, while in the presence of two competitors, development increased and adults lived longer. Aedes albopictus demonstrated a better performance when developing with heterospecifics, with no loss in their development period and improved adult survival. Overall, our results suggest that response to competition can not simply be attributed to the division of resources, and that larvae of both species presented large phenotypic plasticity in their response to the presence or absence of heterospecifics and conspecifics. 相似文献
57.
The first isolation and partial characterization of ovine lentivirus in Czech Republic is described. The virus was isolated
in a tissue culture system derived from plexus choroideus from sheep. The new isolate was compared with prototypic K1514 Maedi-Visna
strain; these two viruses shared antigenic determinants as determined by serological testing. Both viral strains reacted in
a PCR reaction with primers situated in thegag andpol gene. Based on similarities of growth characteristics, antigenic determinants and primer binding sites it can be concluded
that the isolate OPM is an ovine lentivirus and is at least partly related to the prototypic Maedi-Visna strain K1514. 相似文献
58.
Alessandra Bertassoni Guilherme Mourão Rullian César Ribeiro Clarice Silva Cesário Juliana Paula de Oliveira Rita de Cassia Bianchi 《Studies on Neotropical Fauna and Environment》2017,52(1):68-74
This study analyzes a giant anteater’s (Myrmecophaga tridactyla) movement patterns and space use in São Paulo, Brazil. It is the first study to track a giant anteater with Iridium-GPS. The anteater traveled an average distance of 1326 m day–1 with an average speed of 1.04 m min–1. Home range by Kernel was 2.46 km2 while the core area was 0.75 km2, and estimates by Brownian bridge and minimum convex polygon were also provided. The anteater used shrub savanna, open savanna, and water habitats more than expected. Monitoring ended just after 10 days when the female giant anteater’s GPS was found on an illegal trail. 相似文献
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